canine visceral leishmaniasis in kerman, southeast of iran: a seroepidemiological, histopathological and molecular study.
نویسندگان
چکیده
background: canine visceral leishmaniasis (cvl) is a systemic disease with a high mortality rate, caused by a diphasic protozoan parasite, leishmania infantum/chagasi in the world. the objective of the present study was to determine the presence of cvl in the city and suburbs of kerman, using a range of serological, histopathological and molecular methods. methods: blood samples were taken from 80 clinically symptomatic stray dogs all the collected blood samples were tested by direct agglutination test (dat) to detect the anti- leishmania antibodies in dogs, using a cut-off value of ≥1:320. pathological specimens including spleen, liver and lymph nodes were prepared for paraffin blocks, sectioning,staining and final microscopic examination in the pathology laboratory.pcr amplification of kdna from 9 samples of dat positive stray dogs was studied. results: the anti- leishmania antibody was detected in 9 dogs (11.25 %) of the total 80 studied dogs. no significant difference was found between vl infection and gender. in contrast, there was a significant difference between seropositivity and age ( p <0.05). pathological samples showed changes including hyperplasia of infected macrophages and inflammatory cells that occupied sinusoids and splenic cords. among the samples which was characterized by pcr, only one specimen revealed to be mixed infection between l. infantum and l. tropica . conclusion: the results revealed a high prevalence of l. infantum infection in stray dogs in kerman. this kind of information is needed for implementation of future control programs.
منابع مشابه
Canine Visceral Leishmaniasis in Kerman, Southeast of Iran: A Seroepidemiological, Histopathological and Molecular Study
BACKGROUND Canine visceral leishmaniasis (CVL) is a systemic disease with a high mortality rate, caused by a diphasic protozoan parasite, Leishmania infantum/chagasi in the world. The objective of the present study was to determine the presence of CVL in the city and suburbs of Kerman, using a range of serological, histopathological and molecular methods. METHODS Blood samples were taken from...
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متن کامل
Seroprevalence of canine visceral leishmaniasis in southeast of Iran.
Visceral leishmaniasis is an endemic disease in many parts of Iran and infected dogs constitute the main domestic reservoirs that play a key role in transmission to humans. The objective of this study was to assess the seroprevalence of canine visceral leishmaniasis (CVL) by enzyme linked immunosorbent assay (ELISA) in southeast of Iran. This survey was carried out from 2009 to 2011 in Kerman, ...
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BACKGROUND Visceral leishmaniasis (kala-azar) is an endemic disease in some areas of Iran. A cross- sectional study was conducted for sero-epidemiological survey of visceral leishmaniasis (VL) in Baft district from Kerman Province, southeast of Iran. METHODS Blood samples were collected from children up to 12 years old and 10% of adult population from Baft villages with a multi-stage randomiz...
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Clinically infected dogs have been identified as the main reservoir hosts of visceral leishmaniasis (VL)caused by Leishmania infantum. Recently asymptomatic infected dogs were regarded to be as important asclinically ill dogs. This study was aimed to determine the seroprevalence of L. infantum infection amongasymptomatic dogs in the southwest and central parts of Iran and to investigate possibl...
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عنوان ژورنال:
iranian journal of parasitologyجلد ۹، شماره ۳، صفحات ۳۴۲-۳۴۹
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